The museum contains the following collections:
Finds from the salvage excavations carried out by the 20th Ephorate of Prehistoric and Classical Antiquities throughout the island of Lesbos Finds from excavations conducted in collaboration with the Ephorate of Speleology at the Neolithic cave of Agios Bartholomaios Finds from the systematic excavations of the British School of Archaeology at Thermi and Antissa Finds from the activities of the Canadian Archaeological Institute in the city of Mytilene Objects from private donations from the Semanteris, Grimanis, Alatzas, Lagos and Binos collections.